Correlation between vegetation indexes generated at Vitis Vinifera L. and soil, plant and production parameters for emergency application in decision making / Correlação entre indices de vegetação gerados em Vitis Vinifera L. e parâmetros de solo, planta e produção para aplicação emergencial na tomada de decisão

Correlation between proximal sensing techniques and laboratory results of qualitative variables plus agronomic attributes was evaluated of a 3,0 ha vineyard in the county of Muitos Capões, Northeast of Rio Grande do Sul State, Brazil, in Vitis vinifera L. at 2017/2018 harvest, aiming to evaluate the replacement of conventional laboratory analysis in viticulture by Vegetation Indexes, at situations were laboratory access are unavailable. Based on bibliographic research, looking for vegetative indexes developed or used for canopy reflectance analysis on grapevines and whose working bands were within the spectral range provided by the equipment used, a total of 17 viable candidates were obtained. These chosen vegetation indices were correlated, through Pearson (5%), with agronomic soil attributes (apparent electrical conductivity, clay, pH in H2O, phosphorus, potassium, organic matter, aluminum, calcium, magnesium, effective CTC, CTC at pH 7.0, zinc, copper, sulfur and boron) for depths 0 -20 cm and 20-40 cm, and plant tissue (Nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, copper, zinc, iron, manganese and boron) , in addition to some key oenological and phytotechnical parameters for the quantification of wine production and quality. One hundred and thirty ninesignificant correlations were obtained from this cross, with 36 moderate coefficients between 19 parameter variables versus 12 of the indexes. We concluded that in cases where access or availability of laboratory analyzes is difficult or impracticable, the use of vegetation indices is possible if the correlation coefficients reach, at least, the moderate magnitude, serving as a support to decision making until the lack analytical structure to be remedied.

Avaliou-se a correlação entre as técnicas de sensoriamento proximal e os resultados laboratoriais de variáveis qualitativas, mais os atributos agronômicos do solo de um vinhedo de 3,0 ha no município de Muitos Capões, região nordeste do estado do Rio Grande do Sul, Brasil, na safra 2017/2018. Objetivou avaliar a substituição das análises laboratoriais convencionais em viticultura por Índices de Vegetação, em situações de indisponibilidade de acesso ao laboratório. Com base em pesquisa bibliográfica, buscaram-se índices vegetativos desenvolvidos ou utilizados para análise de refletância de dossel em videiras e cujas bandas de trabalho estavam dentro do intervalo espectral fornecido pelo equipamento utilizado, obtendo-se um total de 17 candidatos viáveis. Esses índices de vegetação escolhidos foram correlacionados, por meio de Pearson (5%), com atributos agronômicos do solo (condutividade elétrica aparente, argila, pH em H2O, fósforo, potássio, matéria orgânica, alumínio, cálcio, magnésio, CTC efetivo, CTC em pH 7,0, zinco, cobre, enxofre e boro) para profundidades de 0 - 20 cm e 20 - 40 cm, e tecido vegetal (nitrogênio, fósforo, potássio, cálcio, magnésio, enxofre, cobre, zinco, ferro, manganês e boro), além de alguns parâmetros enológicos e fitotécnicos essenciais para a quantificação da produção e qualidade do vinho. Deste cruzamento foram obtidas 139 correlações significativas, resultando 36 coeficientes moderados entre 19 variáveis de parâmetros versus 12 dos índices. Concluímos que nos casos em que o acesso ou disponibilidade de análises laboratoriais é difícil ou impraticável, a utilização de índices de vegetação é possível, desde que os coeficientes de correlação atinjam, pelo menos, a magnitude moderada, servindo como suporte para a tomada de decisão até a falta de estrutura analítica ser remediada.

Improving micropropagation of some grape cultivars via boron, calcium and phosphate

BACKGROUND: An efficient regeneration protocol is a priority for the successful application of plant biotechnology. Grape nodal explants were used to develop a micropropagation protocol for Thompson Seedless and Taify cvs. Explants were cultured on MS medium supplemented with Kinetin or benzylaminopurine (BA) and indolebutyric acid (IBA). RESULTS: For both cultivars, axillary buds were grown, only, on a medium enriched with kinetin, moreover, shoot tip necrosis and callus formation were observed on Thompson Seedless cv. cultures grown on a medium with BA. Supplementing the growth medium with 100 mM (boron) B and 2.5 mM (calcium) Ca successfully help overcome these phenomena. The highest regenerated shoot numbers (14 and 6.2 explant 1 ) for Taify and Thompson Seedless cvs., respectively, were on media supplemented with 13.2 mM BA + 4.9 mM IBA and BA 13.2 mM + 5.8 mM IBA, respectively. Moreover, these media supported the developing shoots to have the heaviest dry weights (1.46 and 0.72 mg explant 1 ) for Taify and Thompson Seedless cvs., respectively. Thompson Seedless cv. regenerated shoot numbers and their dry weights were significantly increased by increasing the MS medium PO4 concentration. However, these two parameters were significantly decreased for Taify cv. Developing shoots were elongated and rooted on MS medium enriched with 4.9 mM, IBA 100 mM B and 2.5 mM Ca. Plantlets were acclimatized and successfully transferred to the greenhouse conditions. CONCLUSIONS: A novel promising protocol for Thomson Seedless and Taify cvs. micropropagation using single nodes has been developed.

In vitro propagation of Vitis vinifera L. cv. 'Monastrell'

Background: A protocol for the micropropagation of the grape (Vitis vinifera L.) cultivar 'Monastrell' was developed. Initial plant material was obtained from the sanitary selection of grapevine plants performed by real-time RT-PCR to confirm the absence of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine leafroll-associated virus 1, Grapevine leafroll-associated virus 3, and Grapevine fleck virus. Results: The effects of the salt composition (comparing Lloyd and McCown woody plant medium and Murashige and Skoog medium 1/2 macronutrients) and the growth regulator benzylaminopurine (BAP), at 0 and 8.9 µM, on plant propagation were evaluated using nodes as explants. The most efficient procedure consisted of bud induction in the medium with Lloyd and McCown woody plant salts and 8.9 µM BAP for 30 d along with elongation in cytokinin-free medium for 60 d, which gave 22 nodes/explant (174 plants/initial plant). A second cycle of propagation in a medium without BAP for another 60 d could give approximately 10,000 nodes, which can be obtained after an additional 2 months of culture. All plants acclimatized after the second cycle of multiplication were successfully transferred to soil. Conclusion: We developed an optimal protocol for V. vinifera cv. 'Monastrell' micropropagation, the first described for this cultivar.

Phenology, reproductive biology and growing degree days of the grapevine ‘Isabel’ (Vitis labrusca, Vitaceae) cultivated in northeastern Brazil / Fenologia, biologia reprodutiva e graus-dias da videira “Isabel” (Vitis labrusca, Vitaceae) cultivada no nordeste do Brasil

Abstract Phenology and reproductive biology of cultivated species are important for the comprehension of the requirements for fruit and seed production and the management of pollinators. This study aimed to characterise the phenology, reproductive biology and growing degree days of the grapevine ‘Isabel’ (Vitis labrusca) in northeastern Brazil during January 2011 (P1), Augst 2011 (P2), April 2012 (P3) and August 2012 (P4). We recorded the duration (days) of the phenological stages, pruning (P), woolly bud (W), budburst (B), inflorescence development (ID), flowering (F), ripening (R) and harvest (H). We analysed the floral biology, the sexual system and the breeding system. We measured the growing degree days (GDD) required to reach the subperiods P-B, B-F and F-H. The periods P1, P2, P3 and P4 lasted for 116, 125, 117 and 130 days, respectively. The number of days of harvest were similar in the same dry (P1 and P3) and rainy (P2 and P4) periods. All the periods that we recorded were shorter than those observed in other regions of Brazil, which may be attributable to the mean temperature and carbohydrate metabolism. The flowers are green, hermaphroditic, with an odour of mignonette, low pollen viability and autogamous. The base temperature of 10°C was considered the most adequate for the subperiods as has been documented for other grape varieties in Brazil. Thus, temperature was also the most adequate for the cycles, presenting a smaller standard deviation (0.119, 0.147, 0.156 and 0.153 to P1, P2, P3 and P4, respectively) when compared to a base temperature of 12°C (0.122, 0.158, 0.165 and 0.160 to P1, P2, P3 and P4, respectively). The higher and the lower observed GDD were 1972.17 and 1870.05, respectively, both above the values recorded in other parts of Brazil for same variety. The phonological results, including knowledge of growing degree days, are important to the planning of cultures at the study site and in other regions that have similar climatic conditions and make it possible to pre-determine the harvest.

Resumo Fenologia e biologia reprodutiva de espécies cultivadas são importantes para a compreensão dos requerimentos para a produção de frutos e sementes, bem como para o manejo de polinizadores. O presente estudo objetivou caracterizar a fenologia, a biologia reprodutiva e a exigência térmica (graus-dias) da videira “Isabel” (Vitis labrusca) no nordeste do Brasil, durante janeiro/2011 (P1), Agosto/2011 (P2), Abril/2012 (P3) e Agosto/2012 (P4). Analisamos a duração (dias) dos estádios fenológicos poda (PO), gema-algodão (GA), brotamento (BR), aparecimento da inflorescência (AI), florescimento (FL), início da maturação (IM) e colheita (CO). Analisamos a biologia floral, o sistema sexual e o sistema reprodutivo. As exigências térmicas foram obtidas em termos de graus-dia (GD) necessários para atingir os subperíodos PO-BR, BR-FL e FL-CO. A duração do ciclo foi de 116, 125, 117 e 130 dias para as épocas P1, P2, P3 e P4 respectivamente, sendo o número de dias de colheita semelhantes no período seco (P1 e P3) e chuvoso (P2 e P4). Todos os períodos analisados foram mais curtos do que aqueles observados em outras regiões do Brasil, o que pode ser atribuído à temperatura média e ao metabolismo de carboidratos. As flores são verdes, hermafroditas, com odor almiscarado, baixa viabilidade polínica e autógama. A temperatura base de 10ºC foi considerada a mais adequada para os subperíodos e tem sido documentada para outras variedades de uva no Brasil; portanto, a temperatura foi também a mais adequada para os ciclos, apresentando um desvio padrão menor (0,119; 0,147; 0,156 e 0,153 para P1, P2, P3 e P4, respectivamente), comparado com uma temperatura base de 12ºC (0,122; 0,158; 0,165 e 0,160 para P1, P2, P3 e P4, respectivamente). O maior é o menor GDD observado foram 1972,17 e 1870,05, respectivamente. Os resultados fenológicos, incluindo o conhecimento dos graus dias, são importantes para o planejamento de culturas no local e em outras regiões com condições climáticas semelhantes, tornando possível pré-determinar a colheita.

Effect of arbuscular mycorrhizal fungi on young vines in copper-contaminated soil

Abstract High copper (Cu) levels in uprooted old vineyard soils may cause toxicity in transplanted young vines, although such toxicity may be reduced by inoculating plants with arbuscular mycorrhizal fungi (AMF). The objective of this study was to evaluate the effects of AMF on the plant growth, chlorophyll contents, mycorrhizal colonization, and Cu and phosphorus (P) absorption in young vines cultivated in a vineyard soil contaminated by Cu. Commercial vineyard soil with high Cu levels was placed in plastic tubes and transplanted with young vines, which were inoculated with six AMF species (Dentiscutata heterogama, Gigaspora gigantea, Acaulospora morrowiae, A. colombiana, Rhizophagus clarus, R. irregularis) and a control treatment on randomized blocks with 12 replicates. After 130 days, the mycorrhizal colonization, root and shoot dry matter (DM), height increment, P and Cu absorption, and chlorophyll contents were evaluated. The height increment, shoot DM and chlorophyll contents were not promoted by AMF, although the root DM was increased by R. clarus and R. irregularis, which had the greatest mycorrhizal colonization and P uptake. AMF increased Cu absorption but decreased its transport to shoots. Thus, AMF species, particularly R. clarus and R. irregularis, contribute to the establishment of young vines exposed to high Cu levels.

The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures

BACKGROUND: Plant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell suspension cultures of grapevine. Cell suspensions initiated from callus belonging to petiole tissue was used as a plant material. Cadmium chloride was applied to cell suspension cultures in different concentration (1.0 mM and 1.5 mM) to enhance secondary metabolite (total phenolics, total flavanols, total flavonols, trans-resveratrol, and α-, ß-, γ- δ-tocopherols) production. Cells were harvested at two days intervals until the 6th day of cultures. Amounts of total phenolics, total flavanols and total flavonols; trans-resveratrol and tocopherols (α-, ß-, γ- and δ-tocopherols) and dry cell weights were determined in the harvested cells. RESULTS: Phenolic contents were significantly affected by the sampling time and cadmium concentrations. The highest values of total phenolic (168.82 mg/100 g), total flavanol (15.94 mg/100 g), total flavonol (14.73 mg/100 g) and trans-resveratrol (490.76 µg/100 g) were found in cells treated with 1.0 mM CdCl2 and harvested at day 2. Contents of tocopherols in the cells cultured in the presence of 1.0 mM CdCl2 gradually increased during the culture period and the highest values of α, ß and γ tocopherols (145.61, 25.52 and 18.56 µg/100 g) were detected in the cell cultures collected at day 6. CONCLUSIONS: As a conclusion, secondary metabolite contents were increased by cadmium chloride application and sampling time, while dry cell weights was reduced by cadmium chloride treatments.

Physiological studies on duration and types of dormancy in some grape CVS

This study was carried out for two successive seasons on "Flame, Superior and Thompson" Seedless grapevine cultivars grown in sandy soil under drip irrigation system. Dormancy types and periods, changes in endogenous hormones and thickness of scale layer in relation to de-scaling, exposure to various chilling and ODD regimes were investigated, in addition to the thermal requirements for some phenological stages. Results indicated that grape buds exhibit a short shallow dormancy period, Part of it is attributed to Endo-dormancy and other to Para-dormancy which are basically due to the scales. Scale layers diameter increased markedly during the dormancy period. Such increment might restrict bud growth and expansion, it may be a source of ABA. On the other hand, dormancy period of the three considered grape cultivars may depend on the interaction between both chilling and ODD with various degrees. It was clear that scaling increased bud burst percentage in most sampling dates. Results concerning endogenous hormones emphasized general increase in the promoting hormones [IAA and GA3] and general decrease in ABA at post dormancy, whereas the reverse was detected in the deep dormancy. GA3 was not detected at both Pre- and deep dormancy phases

Argentinean cultivars of Vitis vinifera grow better than european ones when cultured in vitro under salinity

Argentinean Vitis vinifera cultivars although originated from Europe, have clear ampeIographic and genotypic differences as compared with the European cultivars currently used in wine making. In vitro evaluation of salt tolerance has been used in many species. Our hypothesis was that Argentinean cultivars are more tolerant to salinity than European ones. Three European cultivars, Malbec, Cabernet Sauvignon and Chardonnay and four Argentinean cultivars, Cereza, Criolla Chica, Pedro Gimenez and Torrontes Riojano were tested by in vitro culture. Treatments included: 1) Control, 2) 60 mEq/L of a mixture of three parts of NaCl and one part of CaCl2 and 3) 90 mEq/L of the salt mixture. Results from two experiments (I and II) are reported. No differences were found in plant survival, expressed as % of the respective control, among cultivars. Leaf area, leaf, stem and total dry matter (DM) in Experiment I and leaf area, leaf number and leaf, stem, root and total DM in Experiment II, were higher in Argentinean cultivars than in European ones. We conclude that Argentinean cultivars show better performance in growing under salinity, especially in the highest salt concentration. Differences among cultivars, inside each group, were found for most of the measured variables.